Engineered natural triterpenoid elicitors derived from domestic rice bran (Oryza sativa L.) for metagenomic BH3 pocket elicitation & cross-kingdom cell death modulation — targeting conserved apoptotic and Toxin-Antitoxin (TA) machinery without synthetic chemical residues.
Anchored by a comprehensive patent portfolio spanning across these vast fields—from agricultural biosecurity and human oncology to broad-spectrum antivirals and closed-loop biomanufacturing—to22s Bio-Systems targets evolutionarily conserved structural allosteric hinges and metagenomic BH3 hydrophobic grooves (Leu-X-X-X-Asp-X-Leu).
Dual-action foliage elicitor spray driving fungal hyphal suicide (Magnaporthe, Phakopsora, Botrytis) and insect sterol auxotrophy disruption (Spodoptera, Aphis, Diabrotica) without synthetic chemical residues.
Allosteric Cycloartanol therapy targeting the human Bcl-2 , overcoming G101V and D103Y resistance mutations in acute myeloid leukemia (AML).
Modulation of host cell membrane lipid rafts and sterol transporters (ABCA2, NPC1) to inhibit viral envelope fusion and neutralize pore-forming bacterial toxins (PFTs).
Supercritical CO2 refining at 69–70 MPa coupled with heterologous yeast feruloyl esterase (FAE) biocatalysis for high-purity, continuous phytosterol production.
Ingested cycloartane sterols competitively bind midgut sterol transporters (SCP-2, NPC1b) and insect apoptotic regulators (Debcl/Buffy), triggering ecdysis failure.
Molecular docking against host plant cycloartenol synthases confirms zero steric engagement, ensuring complete crop seedling and yield safety without foliage burn.
Natural γ-oryzanol from domestic rice bran occurs as a ferulic acid ester of cycloartenol. Continuous supercritical CO2 refining followed by heterologous feruloyl esterase (FAE) biocatalysis cleanly liberates active Cycloartanol alongside free Ferulic Acid.
Rather than discarding the ferulate co-product, our closed-loop process co-formulates it with Cyanidin-3-Glucoside (C3G, purple rice anthocyanin) to form an intermolecular π-π stacked photoprotective & plant defense complex. Free Ferulic Acid serves as a potent endogenous elicitor—activating phenylalanine ammonia-lyase (PAL), reinforcing plant cell wall lignification, and priming Systemic Acquired Resistance (SAR) to synergize with the primary phytosterol active.
to22s Bio-Systems is actively researching and validating our systems pharmacology platform across rare hematologic cancers, oncoviral malignancies, broad agricultural crop protection, and waveform supercritical biomanufacturing. Protected by our proprietary patent portfolio (Patents A01–A27), our platform is open to field co-development and licensing interest.
Targeting Bcl-2 (α5–α8) to overcome G101V and D103Y clinical resistance mutations.
Neutralizing viral anti-apoptotic decoys (BHRF1, vBcl-2) while modulating host cell membrane lipid raft entry portals (ABCA2, NPC1) against viral envelope fusion.
Dual-action bio-identical phytosterol foliage elicitor driving fungal hyphal suicide and insect molting arrest across major row crops and specialty fruits.
Stabilizes yeast vacuolar membranes (S. cerevisiae) preventing autolysis during high-density fermentation, and Waveform-Driven Supercritical Electrostatic Extraction.
Safety Harbor, FL
PI management, OpenMM explicit-solvent MD simulations, platform architecture & target validation.
University Chemical Engineering Core
Supercritical scCO2 bioprocess extraction, micellar foliage spray formulation, particle sizing.
University Analytical Instrumentation Core
High-resolution Agilent LC-MS QToF, NMR cyclopropane structural verification (δ 0.33, 0.55).
Plant Pathology & Entomology
Land-grant testing core executing fungal hyphal suicide panels and insect molting arrest bioassays.
Access our clean, non-markdown Executive Summaries, Phase I Specific Aims, Phase II computational binding trajectory datasets, and patent claim dossiers.